Phenotypic ASTtraining course in May 2022 at DTU, Kgs. Lyngby, Denmark
May 17-18 Phenotypic methods for Salmonella AMR testing
May 19-20 Phenotypic methods for Campylobacter AMR testing
The course is free and the FWD AMR-RefLabCap project, will cover the travel and accommodation costs.
Programme: Salmonella
| Time | Tuesday May 17 | Wednesday May 18 |
|---|---|---|
| 8:30 | Welcome / Introduction | Summary of the previous day, Q/A |
| EU protocol for harmonised monitoring of antimicrobial resistance in human Salmonella and Campylobacter isolates. | Lab exercise A2: Reading of antimicrobial susceptibility tests results incl. ESBL, ampC and carbapenem characterisation. |
|
| EUCAST protocols, guidelines, clinical/epidemiological breakpoints, interpretation and website. | ||
| 10:15 | Coffee break | Coffee break |
| 10:45 | Isolation and phenotypic identification of Salmonella incl. API, conventional serotyping. | Interpretation and discussion of the results of Lab exercise A2. |
| General introduction to antimicrobial susceptibility testing incl. micro- broth dilution (MBD) disk diffusion (DD), and gradient strip test. |
Introduction to quality management systems and quality assurance incl. international standards (CLSI, ISO, Trek). |
|
| Introduction to ESBL, pAmpC and carbapenemase producing Salmonella, incl. phenotypic testing and interpretation of test results. |
What are the data used for - reporting of monitoring data to EpiPulse/ TESSy. |
|
| 12:45 | Lunch | Lunch |
| 13:30 | Lab exercise A1: Antimicrobial susceptibility testing by MIC gradient tests and DD incl. ESBL, pAmpC and carbapenem characterisation. |
Lab exercise C2: Conventional serotyping of Salmonella (2nd H phase) incl. interpretation and discussion of the results. |
| Lab exercise B: Demonstration of MALDI-TOF. |
Detection of antimicrobial resistance genes and prediction of phenotypic resistance by the ResFinder tool. |
|
| - 17:00 | Lab exercise C1: Conventional serotyping of Salmonella (O and 1st H phases). |
Computer exercise A1: Analysis of DNA sequence data using ResFinder to detect AMR genes in Salmonella. |
Programme: Campylobacter
| Time | Thursday May 19 | Friday May 20 |
|---|---|---|
| 8:30 | Welcome / Introduction |
Summary of the previous day, Q/A |
| EU protocol for harmonised monitoring of antimicrobial resistance in human Salmonella and Campylobacter isolates. |
Lab exercise B1: PCR assay for speciation of Campylobacter. |
|
| EUCAST protocols, guidelines, clinical/epidemiological breakpoints, interpretation and website. |
Interpretation and discussion of the results of Lab exercise B2. |
|
| 10:15 | Coffee break | Coffee break |
| 10:45 | Isolation and phenotypic identification of Campylobacter incl. conventional hippurate hydrolysis and indoxyl acetate tests. | Introduction to quality management systems and quality assurance incl. international standards (CLSI, ISO, Trek). |
| General introduction to antimicrobial susceptibility testing incl. micro- broth dilution (MBD) disk diffusion (DD), and gradient strip test. |
What are the data used for - reporting of monitoring data to EpiPulse/ TESSy. |
|
| Introduction to a PCR assay for speciation of Campylobacter. |
||
| 12:45 | Lunch | Lunch |
| 13:30 | Lab exercise A1: Antimicrobial susceptibility testing by DD and MIC. |
Lab exercise A2: Reading antimicrobial susceptibility test results. |
| Lab exercise C1: Conventional speciation of Campylobacter by conventional hippurate hydrolysis and indoxyl acetate tests. |
Interpretation and discussion of the results of Lab exercise A2. |
|
| Detection of antimicrobial resistance genes and prediction of phenotypic resistance by the ResFinder tool. |
||
| - 17:00 | Lab exercise B1: PCR assay for speciation of Campylobacter. |
Computer exercise A1: Analysis using ResFinder to detect AMR genes in Campylobcater. |